In the culture with glycine at the optimal concentration of 150 mM, the α-CGTase activity in the culture medium reached 23.5 U/mL at 40 h of culture, which was 11-fold higher than that of the culture in regular TB medium. A 2.3-fold increase in the maximum extracellular productivity of recombinant α-CGTase was also observed.

Figure 2 Time courses of the biomass concentration of Bacillus sp. G1 in continuous culture at various dilution rates. After batch culture for 12h, continuous culture was operated at 35oC with aeration of 1v/v/m and agitation at 150rpm for 72h. - "Impact of dilution rate on CGTase activity and productivity from an alkalophilic Bacillus sp. G1 in continuous culture"

In enzymology, a cyclomaltodextrin glucanotransferase (also cyclodextrin glycosyl transferase or CGTase for short) (EC 2.4.1.19) is an enzyme that catalyzes the chemical reaction of cyclizing part of a 1,4-alpha-D-glucan molecule through the formation of a 1,4-alpha-D-glucosidic bond. A CGTase with high coupling activity using γ-cyclodextrin isolated from a novel strain clustering under the genus Carboxydocella. Gulshan Kazi, Zubaida LU; Lundemo, Pontus LU; Fridjonsson, Olafur H; Hreggvidson, Gudmundur O; Adlercreutz, Patrick LU and Nordberg Karlsson, Eva LU () In Glycobiology 25 (5). p.514-523 Activity and stability characteristics of an alkaline active cyclodextrin glycosyltransferase (CGTase) enzyme from the alkaliphilic Bacillus agaradhaerens LS-3C strain are reported. The enzyme displays unusually high amylolytic activity in relation to the cyclization activity.

Cgtase activity

And the target prod- 2.3. Assay of enzyme activity CGTase activity was assayed as described by Kato and Horikoshi [5]. One unit of enzyme activity was de¢ned as the amount of enzyme that forms 1 Wmol of Q-CD from soluble starch in 1 min. Alkaline phos-phatase was assayed by the method of Yamane et al. [9]. One unit of alkaline phosphatase activity is de- In the culture with glycine at the optimal concentration of 150 mM, the α-CGTase activity in the culture medium reached 23.5 U/mL at 40 h of culture, which was 11-fold higher than that of the culture in regular TB medium.

nitrogen source for CGTase production. Ca2+ influences the CGTase production and Zn2+ inhibits the enzyme. High CGTase activity was observed at 24 h of.

a-CD does not contain any CGTase activity because the enzyme is . inactivated by heat and is removed completely during the a-CD production . process.

Cyclodextrin glucanotransferases (CGTases; EC 2.4.1.19) have mainly been characterized for their ability to produce cyclodextrins (CDs) from starch in an intramolecular transglycosylation reaction (cyclization).

689 av J Wouters · 2005 · Citerat av 1 — cgt, CGTase, Differential display, Symbiosis, Nostoc, Gunnera and Gunnera sp. mucilage could not induce nitrogenase activity in darkness. Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; CGTase; cyclodextrin glycosyltransferase; alkyl glycoside; enzyme stabliity; 2 344 4 a- Bacillus megaterium B. macerans CGTase 22 C-2, C-3, C-4 5) A. B., Lapa, A. J., Pharmacological evaluation of the anti-inflammatory activity of a 24 cost | company | product | cost management | activity | sul fab | cgtase | fed-batch cultivation | biolector | dera | autodisplay | enbase. As suggested by activity analysis, expression levels might have been increased in mutated compared to wild-type CGTases in the strains containing the RNaseE product | cost management | activity | sul | agribusiness | environmental cost fab | cgtase | fed-batch cultivation | biolector | dera | autodisplay | enbase 844 C-niveau - KVUC Bild. EP2316929B1 - Maltogenic alpha-amylase variants - Google Patents. Effect of storage temperature on -amylase activity from . Illias Research PapersThe starting gene-protein is a variant of CGTase Bacillus sp.

The CGTase production was further studied with the optimized process parameters on submerged cultivations (SC) and solid-state cultivations (SSC) using soybean industrial fibrous residue (SIFR). The maximum CGTase activity obtained on SC was 1,155 U mL−1 under aerobic conditions. Cell growth and CGTase synthesis in SSC using SIFR as substrate After three rounds of mutagenesis the hydrolytic activity had increased 90-fold, reaching the highest hydrolytic activity ever reported for a CGTase. The single mutation with the largest effect (A230V) occurred in a residue not studied before. 2017-02-20 · α-CGTase activity.
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Maximum CGTase activity was obtained atlow dilution rate range (0.03-0.07h-1). CGTase activity decreased from 26.4 to 12.4 U/mlwhen the dilution rate was increased to 0.3h-1… CONTINUE READING Enzyme activity of CGTase from Alkalophilic Bacillus sp. BL-31 was stimulated by presence of Mn 2+ which resulted in increased yield of glycosylated naringin from 80.2% to 92.1% .

- "Impact of dilution rate on CGTase activity and productivity from an alkalophilic Bacillus sp. G1 in continuous culture" Some of the enzyme activity was also observed in the periplasmic and the intracellular fractions. When the mature CGTase G1 gene (without signal peptide) was cloned into pQE and pWH expression vectors and transformed into E. coli, almost all of the CGTase activity was detected intracellularly (data not shown).
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CGTase can catalyse four reactions: cyclization, coupling, disproportionation, and hydrolysis (Li et al., 2014b), though the hydrolysis activity is relatively weak (Costa et al., 2009). CGTase is a member of glycoside hydrolase family 13 (GH13) (Buchholz & Seibel, 2008 ; Stam et al., 2006 ).

For partial purification, the enzyme was 15 Nov 2013 stability compared with free enzyme. The optimum pH for enzyme activity was pH 8 and pH 7.5 for free and immobilized CGTase, respectively, A CGTase with high coupling activity using γ-cyclodextrin isolated from a novel strain clustering under the genus Carboxydocella. This page in English.